Initiating Method Development with Scouting Gradients—Where to Begin and How to Proceed?

With so many options for method parameters to adjust during development, identifying a starting point can be intimidating. Starting with scouting gradients simplify the process, and yield rich results that inform subsequent development steps. In this installment, we discuss how design gradients, use resulting data make decisions about next steps (including whether isocratic or gradient elution), improve separation once an elution mode has been chosen.